Publications including proceedings are HERE (Japanese page)
Presentations are HERE (Japanese page)

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  1. Channel Current Analysis Estimates the Pore-formation and the Penetration of Transmembrane Peptides
    Yusuke Sekiya, Shungo Sakashita Keisuke Shimizu, Kenji Usui, Ryuji Kawano
    Analyst, 143, 3540-3543
    DOI: 10.1039/C8AN00243F
    [Journal page] [Pubmed] [Inside cover]

  2. Applications of a Novel Biodetection System to Saliva Using Protein Fingerprints with Data Processing
    Yuki Tominaga, Kenji Usui, Akiyoshi Hirata, Hiro-O Ito, Kiyoshi Nokihara
    Bioorganic & Medicinal Chemistry, 26, 3210-3216
    DOI: 10.1016/j.bmc.2018.04.049
    [Journal page] [PubMed]

  3. Editorial: Organic-Inorganic Hybrid Materials and Their Applications
    Kin-ya Tomizaki, Yoshio Hamada, Kenji Usui
    Protein & Peptide Letters, 25, 2-3
    DOI: 10.2174/092986652501180418150844
    [Journal Page]

  4. [Pubmed]
  5. Peptides for Silica Precipitation: Amino Acid Sequences for Directing Mineralization
    Makoto Ozaki, Shungo Sakashita, Yoshio Hamada, Kenji Usui*
    Protein & Peptide Letters, 25, 15-24
    DOI: 10.2174/0929866525666171214111007
    [Journal page] [Pubmed]

  6. Modification of the N-Terminus of a Calcium Carbonate Precipitating Peptide Affects Calcium Carbonate Mineralization
    Kenji Usui*, Shin-ichiro Yokota, Makoto Ozaki, Shungo Sakashita, Takahito Imai, Kin-ya Tomizaki
    Protein & Peptide Letters, 25, 42-47
    DOI: 10.2174/0929866525666171221114658
    [Journal page] [Pubmed]

  7. Design of Anti-Alzheimer's Disease Agents Focusing on a Specific Interaction with Target Biomolecules
    Yoshio Hamada*, Kenji Usui
    Neuromethods, 132, 207-228
    DOI: 10.1007/978-1-4939-7404-7
    [Journal page]



  1. DNA G-Wire Formation Using an Artificial Peptide is Controlled by Protease Activity
    Kenji Usui*, Arisa Okada, Shungo Sakashita, Masayuki Shimooka, Takaaki Tsuruoka, Shu-ichi Nakano, Daisuke Miyoshi, Tsukasa Mashima, Masato Katahira, Yoshio Hamada
    Molecules, 22, 1991
    DOI: 10.3390/molecules22111991
    [Journal page] [PubMed]

  2. Non-Covalent Loading of Anti-Cancer Doxorubicin by Modularizable Peptide Self-Assemblies for a Nanoscale Drug Carrier
    Kin-ya Tomizaki*, Kohei Kishioka, Shunsuke Kataoka, Makoto Miyatani, Takuya Ikeda, Mami Komada, Takahito Imai, Kenji Usui*
    Molecules, 22, 1916
    DOI: 10.3390/molecules22111916
    [Journal page] [PubMed]

  3. Fluorescent and Luminescent Fusion Proteins for Analyses of Amyloid Beta Peptide Aggregation.
    Kenji Usui, Masayasu Mie, Takashi Andou, Hisakazu Mihara, Eiry Kobatake
    J Pept Sci, 23, 659-665
    DOI: 10.1002/psc.3003
    [Journal page] [PubMed]



  1. Site-Specific Control of Multiple Mineralizations Using a Designed Peptide and DNA
    Kenji Usui*, Makoto Ozaki, Aoi Yamada, Yoshio Hamada, Takaaki Tsuruoka, Takahito Imai, Kin-ya Tomizaki
    Nanoscale, 8, 17081-17084 ※Inside back cover feature
    DOI: 10.1039/c6nr03468c

    [Journal page] [PubMed] [Inside back cover]

  2. Site-Specific Control of Silica Mineralization on DNA Using a Designed Peptide
    Makoto Ozaki, Kazuma Nagai, Hiroto Nishiyama, Takaaki Tsuruoka, Satoshi Fujii, Tamaki Endoh, Takahito Imai, Kin-ya Tomizaki, Kenji Usui*
    Chem. Commun., 52, 4010-4013 ※Inside back cover feature
    DOI: 10.1039/c5cc07870a
    [Journal page] [PubMed] [Inside back cover]

  3. A Cell Microarray Format: A Peptide Release System Using a Photo-Cleavable Linker for Cell Toxicity and Cell Uptake Analysis
    Kenji Usui*, Kin-ya Tomizaki, Hisakazu Mihara
    Methods. Mol. Biol., 1352, 199-210
    DOI: 10.1007/978-1-4939-3037-1_15
    [Journal page] [PubMed]



  1. Label and Label-Free Detection Techniques for Protein Microarrays
    Amir Syahir, Kenji Usui*, Kin-ya Tomizaki, Kotaro Kajikawa, Hisakazu Mihara
    Microarrays, 4, 228-244
    DOI: 10.3390/microarrays4020228
    [Journal page] [PubMed]

  2. Control of Guanine-Rich DNA Secondary Structures Depending on the Protease Activity Using a Designed PNA Peptide
    Kenji Usui*, Arisa Okada, Keita Kobayashi, Naoki Sugimoto
    Org. Biomol. Chem., 13, 2022-2025
    DOI: 10.1039/c4ob02535k
    [Journal page] [PubMed]

  3. ATP-Binding Peptide-Hydrogel Composite Synthesized by Molecular Imprinting on Beads
    Ayana Takata, Kenji Usui, Jun Matsui
    Molecular Imprinting, 3, 65-70
    [Journal page]



  1. Cellular Differentiation Assessments by Measuring the Degree of Cellular Internalization and Membrane Adsorption Using Designed Peptides.
    Kenji Usui*, Takuya Kikuchi, Kunio Kikuchi, Masayasu Mie, Eiry Kobatake, Hisakazu Mihara
    Bioorg. Med. Chem. lett., 24, 4129-4131
    DOI: 10.1016/j.bmcl.2014.07.053
    [Journal page] [PubMed]

  2. Peptides Targeting G-Quadruplex Structures
    Arisa Okada, Kenji Usui*
    Chemical Biology of Nucleic Acids:Fundamentals and Clinical Applications, 459-475
    [Journal page]



  1. A Novel Array Format for Monitoring Cellular Uptake Using a Photo-Cleavable Linker for Peptide Release
    Kenji Usui*, Takuya. Kikuchi, Kin-ya Tomizaki, Takashi Kakiyama, Hisakazu Mihara
    Chem. Commun., 49, 6394-6396
    DOI: 10.1039/c3cc41632a
    [Journal page] [PubMed]

  2. Systematic Screening of the Cellular Uptake of Designed Alpha-Helix Peptides
    Kenji Usui*, Takuya. Kikuchi, Masayasu Mie, Eiry Kobatake, Hisakazu Mihara
    Bioorg. Med. Chem., 21, 2560-2567
    DOI: 10.1016/j.bmc.2013.02.030
    [Journal page] [PubMed]

  3. A Peptide Release System Using a Photo-Cleavable Linker in a Cell Array Format for Cell-Toxicity Analysis
    Takashi Kakiyama, Kenji Usui*, Kin-ya Tomizaki, Masayasu Mie, Eiry Kobatake, Hisakazu Mihara
    Polym J., 45, 535-539
    [Journal page]



  1. Use of a Designed Peptide Library to Screen for Binders to a Particular DNA G-Quadruplex Sequence
    Keita Kobayashi, Noriko Matsui, Kenji Usui*
    J. Nucleic Acids , 2011:572873
    DOI: 10.4061/2011/572873
    [Journal page] [PubMed]

  2. Cell Fingerprint Patterns Using Designed α-Helical Peptides to Screen for Cell-Specific Toxicity
    Kenji Usui, Takashi Kakiyama, Kin-ya Tomizaki, Masayasu Mie, Eiry Kobatake, Hisakazu Mihara
    Bioorg. Med. Chem. Lett., 21, 6281-6284
    DOI: 10.1016/j.bmcl.2011.09.002
    [Journal page] [PubMed]

  3. Thermodynamic Stability of Hoogsteen and Watson-Crick Base Pairs in the Presence of Histone H3-Mimicking Peptide
    Smritimoy Pramanik, Kaori Nakamura, Kenji Usui, Shu-ichi Nakano, Sarika Saxena, Jun Matsui, Daisuke Miyoshi, Naoki Sugimoto
    Chem. Commun., 47, 3967-3971
    DOI: 10.1039/c0cc05776b
    [Journal page] [PubMed]



  1. Protein-protein Interactions and Selection: Array-Based Techniques for Screening Disease-Associated Biomarkers in Predictive/Early Diagnosis
    Kin-ya Tomizaki, Kenji Usui, Hisakazu Mihara
    FEBS J., 277, 1996-2005
    DOI: 10.1111/j.1742-4658.2010.07626.x.
    [Journal page] [PDF] [PubMed]



  1. Site-Specific Modification of Alzheimer's Peptides by Cholesterol Oxidation Products Enhances Aggregation Energetics and Neurotoxicity
    Kenji Usui, John D. Hulleman, Johan F. Paulsson, Sarah J. Siegel, Evan T. Powers, Jeffery W. Kelly
    Proc. Natl. Acad. Sci. USA, 106, 18563-18568
    DOI: 10.1073/pnas.0804758106
    [Journal page] [PDF] [PubMed]

  2. A Designed Peptide Chip: Protein Fingerprinting Technology with a Dry Peptide Array and Statistical Data Mining
    Kenji Usui, Kin-ya Tomizaki, Hisakazu Mihara
    Methods. Mol. Biol., 570, 273-284
    DOI: 10.1007/978-1-60327-394-7_13
    [Journal page] [PubMed]

  3. Proteins: Array-Based Techniques
    Kin-ya Tomizaki, Kenji Usui, Hisakazu Mihara
    Wiley Encyclopedia of Chemical Biology, 4, 144-158
    DOI: 10.1002/9780470048672.wecb022
    [Journal page]



  1. Screening of Alpha-Helical Peptide Ligands Controlling a Calcineurin-Phosphatase Activity
    Kenji Usui, Kin-ya Tomizaki, Hisakazu Mihara
    Bioorg. Med. Chem. Lett., 17, 167-171
    DOI: 10.1016/j.bmcl.2006.09.075
    [Journal page] [PubMed]

  2. Interactions between Peptides Containing Nucleobase Amino Acids and T7 Phages Displaying S. Cerevisiae Proteins
    Sinya Watanabe, Kin-ya Tomizaki, Tsuyoshi Takahashi, Kenji Usui, Kotaro Kajikawa, Hisakazu Mihara
    Biopolymers, 88, 131-140
    DOI: 10.1002/bip.20662
    [Journal page] [PubMed]



  1. Protein-Fingerprint Data Mining of a Designed Alpha-Helical Peptide Array
    Kenji Usui, Kin-ya Tomizaki, Hisakazu Mihara
    Mol. BioSyst., 2, 417-420 ※Inside back cover feature
    [Journal page] [PubMed]

  2. A Novel Peptide Microarray for Protein Detection and Analysis Utilizing a Dry Peptide Array System
    Kenji Usui, Kin-ya Tomizaki, Takafumi Ohyama, Kiyoshi Nokihara, Hisakazu Mihara
    Mol. BioSyst., 2, 113-121
    DOI: 10.1039/b514263f
    [Journal page] [PubMed]

  3. A PNA-DNA Hybridization Chip Approach for the Detection of Beta-Secretase Activity
    Shusuke Sano, Kin-ya Tomizaki, Kenji Usui, Hisakazu Mihara
    Bioorg. Med. Chem. Lett., 16, 503-506
    DOI: 10.1016/j.bmcl.2005.10.064
    [Journal page] [PubMed]



  1. A Designed Glycopeptide Array for Characterization of Sugar-Binding Proteins toward a Glycopeptide Chip Technology
    Kenji Usui, Tetsunori Ojima, Kin-ya Tomizaki, Hisakazu Mihara
    Nanobiotechnology, 1, 191-200
    [Journal page]

  2. IR Study on Stacking Manner of Peptide Nanorings in Peptide Nanotubes
    Yukiko Nagai, Tsutomu Nakanishi, Hajime Okamoto, Kyozaburo Takeda, Yukio Furukawa, Kenji Usui, Hisakazu Mihara
    Jpn. J. Appl. Phys., 44, 7654-7661
    [Journal page]

  3. Difference in Self-Assembling Morphology of Peptide Nanorings
    Hajime Okamoto, Tetsuo Yamada, Hiroshi Miyazaki, Tsutomu Nakanishi, Kyozaburo Takeda, Kenji Usui,Ikuo Obataya, Hisakazu Mihara, Hiroaki Azehara, Wataru Mizutani, Katsushi Hashimoto, Hiroshi Yamaguchi, Yoshiro Hirayama
    Jpn. J. Appl. Phys., 44, 8240-8248
    [Journal page]

  4. Anomalous Reflection of Gold Applicable for a Practical Protein-Detecting Chip Platform
    Sinya Watanabe, Kenji Usui, Kin-ya Tomizaki, Kotaro Kajikawa, Hisakazu Mihara
    Mol. BioSyst., 1, 363-365
    DOI: 10.1039/b513075c
    [Journal page] [PubMed]

  5. Protein-Detecting Microarrays: Current Accomplishments and Requirements
    Kin-ya Tomizaki, Kenji Usui, Hisakazu Mihara
    ChemBioChem, 6, 782-799 ※Inside back cover feature
    DOI: 10.1002/cbic.200400232
    [Journal page] [PubMed]



  1. Peptide Arrays with Designed Alpha-Helical Structures for Characterization of Proteins from FRET-Fingerprint Patterns
    Kenji Usui, Mizuki Takahashi, Kiyoshi Nokihara, Hisakazu Mihara
    Mol. Divers., 8, 209-218
    [Journal page] [PubMed]

  2. Peptide Arrays with Designed Secondary Structures for Protein Characterization Using Fluorescent Fingerprint Patterns
    Kenji Usui, Tetsunori Ojima, Mizuki Takahashi, Kiyoshi Nokihara, Hisakazu Mihara
    Biopolymers, 76, 129-139
    DOI: 10.1002/bip.10568
    [Journal page] [PubMed]

  3. Development of Peptide-Chips Focusing on a High Throughput Protein-Detection System
    Kiyoshi Nokihara, Takafumi Ohyama, Kenji Usui, Koichi. Yoneyama, Mizuki Takahashi, Hisakazu Mihara
    Innovation and Perspectives in Solid Phase Synthesis & Combinatorial Libraries, 83-88